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利用基因组特异性引物和Cas12a介导技术快速检测稻瘟菌小麦致病型 Article
康厚祥, 彭烨, Kangyu Hua, 邓雨飞, Maria Bellizzi, Dipali Rani Gupta, Nur Uddin Mahmud, Alfredo S. Urashima, Sanjoy Kumar Paul, Gary Peterson, 周益林, 周雪平, Md Tofazzal Islam, 王国梁
《工程（英文）》 2021年 第7卷 第9期 页码 1326-1335 doi: 10.1016/j.eng.2020.07.016
Mobile CRISPR-Cas9 based anti-phage system in E. coli
《化学科学与工程前沿（英文）》 2022年 第16卷 第8期 页码 1281-1289 doi: 10.1007/s11705-022-2141-7
关键词： phage infections anti-phage CRISPR-Cas9 conjugative transfer synthetic biology
Advances of 12th CAPS research symposium: young chemists and chemical engineers fronts
Guoping Hu, Baiqian Dai
《化学科学与工程前沿（英文）》 2021年 第15卷 第3期 页码 461-463 doi: 10.1007/s11705-020-2026-6
Introduction to the special section on the 12
Zhen-Min CHENG
《化学科学与工程前沿（英文）》 2009年 第3卷 第1期 页码 1-1 doi: 10.1007/s11705-009-0003-1
用于分枝杆菌碱基编辑的PAM扩展型嗜热链球菌Cas9 C到T和C到G碱基编辑器 Article
张洪源, 张翼飞, 王卫晓, 陈未中, 张侠, 黄行许, 陈伟, 季泉江
《工程（英文）》 2022年 第15卷 第8期 页码 67-77 doi: 10.1016/j.eng.2022.02.013
ThMn12型永磁合金 Review
G.C. Hadjipanayis, A.M. Gabay, A.M. Schönhöbel, A. Martín-Cid, J.M. Barandiaran, D. Niarchos
《工程（英文）》 2020年 第6卷 第2期 页码 140-146 doi: 10.1016/j.eng.2018.12.011
具有四方ThMn12型结构的富铁化合物有潜力满足当下人们对于高磁能积、工作温度为150~200 ℃的贫稀土永磁体的需求。尽管磁体制备技术的发展滞后于相关磁性材料的研究是正常的，但对于ThMn12型磁性材料而言，这种研究进展的差异非常显著。人们发现，引入镧（La）可以提高Sm(Fe,Ti)12中少数低熔点相的稳定性，从而首次使得液相烧结成为可能。然而，La金属的高反应活性会明显地破坏材料的矫顽力（Hc）。,Sm)(Fe,Ti)12合金中也可以实现。机械化学合成的(Sm,Zr)(Fe,Si)12和(Sm,Zr)(Fe,Co,Ti)12粉末可能适合烧结成具有高强度的全致密磁体，尽管在此之前，两者都已开发出较高的各向异性
关键词： 永磁体，稀土永磁体，ThMn 12 结构
CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells
null
《医学前沿（英文）》 2017年 第11卷 第4期 页码 554-562 doi: 10.1007/s11684-017-0543-6
T cells engineered with chimeric antigen receptor (CAR) have been successfully applied to treat advanced refractory B cell malignancy. However, many challenges remain in extending its application toward the treatment of solid tumors. The immunosuppressive nature of tumor microenvironment is considered one of the key factors limiting CAR-T efficacy. One negative regulator of T cell activity is lymphocyte activation gene-3 (LAG-3). We successfully generated LAG-3 knockout T and CAR-T cells with high efficiency using CRISPR-Cas9 mediated gene editing and found that the viability and immune phenotype were not dramatically changed during in vitro culture. LAG-3 knockout CAR-T cells displayed robust antigen-specific antitumor activity in cell culture and in murine xenograft model, which is comparable to standard CAR-T cells. Our study demonstrates an efficient approach to silence immune checkpoint in CAR-T cells via gene editing.
关键词： CAR-T CRISPR-Cas9 LAG-3
Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG
《农业科学与工程前沿（英文）》 2015年 第2卷 第3期 页码 242-248 doi: 10.15302/J-FASE-2015059
Growth suppression of colorectal cancer expressing S492R EGFR by monoclonal antibody CH12
Qiongna Dong, Bizhi Shi, Min Zhou, Huiping Gao, Xiaoying Luo, Zonghai Li, Hua Jiang
《医学前沿（英文）》 2019年 第13卷 第1期 页码 83-93 doi: 10.1007/s11684-019-0682-z
Colorectal cancer (CRC) is a common malignant tumor in the digestive tract, and 30%–85% of CRCs express epidermal growth factor receptors (EGFRs). Recently, treatments using cetuximab, also named C225, an anti-EGFR monoclonal antibody, for CRC have been demonstrated to cause an S492R mutation in EGFR. However, little is known about the biological function of S492R EGFR. Therefore, we attempted to elucidate its biological function in CRC cells and explore new treatment strategies for this mutant form. Our study indicated that EGFR and S492R EGFR accelerate the growth of CRC cells in vitro and in vivo and monoclonal antibody CH12, which specifically recognizes an EGFR tumor-specific epitope, can bind efficiently to S492R EGFR. Furthermore, mAb CH12 showed significantly stronger growth suppression activities and induced a more potent antibody-dependent cellular cytotoxicity effect on CRC cells bearing S492R EGFR than mAb C225. mAb CH12 obviously suppressed the growth of CRC xenografts with S492R EGFR mutations in vivo. Thus, mAb CH12 may be a promising therapeutic agent in treating patients with CRC bearing an S492R EGFR mutation.
关键词： S492R EGFR ectodomain mutation colorectal cancer mAb CH12 immunnotherapy
张果虎,肖清华,马飞
《中国工程科学》 2023年 第25卷 第1期 页码 68-78 doi: 10.15302/J-SSCAE-2023.01.002
具有异构时延的多智能体系统的领导-跟随同步 Research Articles
Branislav REH罧, Volodymyr LYNNYK
《信息与电子工程前沿（英文）》 2021年 第22卷 第1期 页码 1-140 doi: 10.1631/FITEE.2000207
关键词： 多智能体系统；时滞；线性矩阵不等式
Cehuang FU, Shuiyun SHEN, Ruofei WU, Xiaohui YAN, Guofeng XIA, Junliang ZHANG
《能源前沿（英文）》 2022年 第16卷 第4期 页码 607-612 doi: 10.1007/s11708-021-0798-0
关键词： Li4Ti5O12 phenolic-resin-based carbon mesoporous composite graphene
Poor adherence to P2Y12 antagonists increased cardiovascular risks in Chinese PCI-treated patients
null
《医学前沿（英文）》 2017年 第11卷 第1期 页码 53-61 doi: 10.1007/s11684-017-0502-2
Low adherence to secondary prevention medications (ATM) of patients after acute coronary syndrome (ACS) is associated with poor clinical outcomes. However, literature provides limited data on assessment of ATM and risks associated with poor in Chinese patients with ACS. In the current work, ATM was assessed in consecutively recruited patients with ACS in Tongji Hospital from November 5, 2013 to December 31, 2014. A total of 2126 patients were classified under low adherence (proportion of days covered (PDC)<50%) and high adherence (PDC>50%) groups based on their performance after discharge. All patients were followed up at the 1st, 6th, and 12th month of discharge while recording ATM and major adverse cardiac events (MACE). Bivariate logistic regression was used to identify the factors associated with ATM. Cox regression was used to analyze the association between ATM and MACE within one year after discharge. Results showed that coronary artery bypass grafting (CABG) alone had significantly lower proportion of high adherence to P2Y12 antagonists (83.0% vs. 90.7%, P<0.01) than patients treated with percutaneous coronary intervention (PCI) only. Moreover, in patients undergoing PCI, high adherence to P2Y12 antagonists decreased the risk of MACE (hazard ratio=0.172, 95% confidence interval: 0.039–0.763; P=0.021). In conclusion, PCI-treated patients are more prone to remaining adherent to medications than CABG-treated patients. High adherence to P2Y12 antagonists was associated with lower risk of MACE.
关键词： acute coronary syndromes adherence to secondary prevention medications clinical outcome
A PLATFORM TO AID SELECT THE OPTIMAL TOOL TO DESIGN GUIDE RNAS
《农业科学与工程前沿（英文）》 页码 296-305 doi: 10.15302/J-FASE-2022479
● Summaries on sgRNAs design.
标题 作者 时间 类型 操作
利用基因组特异性引物和Cas12a介导技术快速检测稻瘟菌小麦致病型
康厚祥, 彭烨, Kangyu Hua, 邓雨飞, Maria Bellizzi, Dipali Rani Gupta, Nur Uddin Mahmud, Alfredo S. Urashima, Sanjoy Kumar Paul, Gary Peterson, 周益林, 周雪平, Md Tofazzal Islam, 王国梁
期刊论文
Advances of 12th CAPS research symposium: young chemists and chemical engineers fronts
Guoping Hu, Baiqian Dai
期刊论文
ThMn12型永磁合金
G.C. Hadjipanayis, A.M. Gabay, A.M. Schönhöbel, A. Martín-Cid, J.M. Barandiaran, D. Niarchos
期刊论文
Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330
Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG
期刊论文
Growth suppression of colorectal cancer expressing S492R EGFR by monoclonal antibody CH12
Qiongna Dong, Bizhi Shi, Min Zhou, Huiping Gao, Xiaoying Luo, Zonghai Li, Hua Jiang
期刊论文
Facile controlled synthesis of hierarchically structured mesoporous Li4Ti5O12/C/rGO composites as high-performance
Cehuang FU, Shuiyun SHEN, Ruofei WU, Xiaohui YAN, Guofeng XIA, Junliang ZHANG
期刊论文
Poor adherence to P2Y12 antagonists increased cardiovascular risks in Chinese PCI-treated patients
null
期刊论文
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